DNA
Part:BBa_J100141
Designed by: Catherine Doyle Group: Campbell M Lab (2014-04-17)
Theophylline Riboswitch 5
This part combines Theophylline Riboswitch 5 (Part J100137) and superfolder GFP optimized for E. coli (Part J100070). It was assembled using the Golden Gate assembly Method and the BsaI sites that were engineered into the riboswitch and the superfolder GFP.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 3083
Illegal suffix found in sequence at 877 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 3083
Illegal SpeI site found at 878
Illegal PstI site found at 892
Illegal NotI site found at 885
Illegal NotI site found at 3089 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3083
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 3083
Illegal suffix found in sequence at 878 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 3083
Illegal XbaI site found at 3098
Illegal SpeI site found at 878
Illegal PstI site found at 892
Illegal AgeI site found at 100
Illegal AgeI site found at 181 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 169
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Categories
Parameters
None |